https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Oxidative Stress and Reproductive Function: Oxidative stress and the long-term storage of horse spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:53340 Wed 22 Nov 2023 10:18:49 AEDT ]]> Assisted breeding technology in the saltwater crocodile Crocodylus porosus: a review and look to the future https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43523 Wed 21 Sep 2022 11:25:36 AEST ]]> Evaluating amphibian biobanking and reproduction for captive breeding programs according to the Amphibian Conservation Action Plan objectives https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46347 Wed 16 Nov 2022 08:47:07 AEDT ]]> Advances in assisted reproductive techniques for the conservation of Australian carnivorous marsupials https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5839 Wed 11 Apr 2018 10:16:08 AEST ]]> Towards gene banking amphibian maternal germ lines: short-term incubation, cryoprotectant tolerance and cryopreservation of embryonic cells of the frog, limnodynastes peronii https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14928 Wed 11 Apr 2018 10:08:24 AEST ]]> The Increasing Role of Short-Term Sperm Storage and Cryopreservation in Conserving Threatened Amphibian Species https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51284 Wed 06 Mar 2024 15:39:43 AEDT ]]> Integrating biobanking could produce significant cost benefits and minimise inbreeding for Australian amphibian captive breeding programs https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44935 Tue 25 Oct 2022 10:36:39 AEDT ]]> Sperm collection and storage for the sustainable management of amphibian biodiversity https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37426 Tue 17 Nov 2020 10:22:21 AEDT ]]> Generation of a sexually mature individual of the Eastern dwarf tree frog, Litoria fallax, from cryopreserved testicular macerates: proof of capacity of cryopreserved sperm derived offspring to complete development https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34867 Tue 03 Sep 2019 18:01:56 AEST ]]> Analysis of sperm separation protocols for isolating cryopreserved human spermatozoa. https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50661 Tue 01 Aug 2023 13:06:32 AEST ]]> Modeling genetic benefits and financial costs of integrating biobanking into the conservation breeding of managed marsupials https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50548 Tue 01 Aug 2023 10:55:18 AEST ]]> Sperm cryopreservation: current status and future developments https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50551 Tue 01 Aug 2023 10:53:37 AEST ]]> Cell culture and karyotypic description of Pseudophrynecoriacea (Keferstein, 1868) (Amphibia, Anura) from the New South Wales Central Coast https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:53986 Thu 25 Jan 2024 13:04:11 AEDT ]]> Amphibian declines in the twenty-first century: why we need assisted reproductive technologies https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17628 Thu 18 Jun 2015 09:53:25 AEST ]]> Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8358 Sat 24 Mar 2018 08:39:51 AEDT ]]> Artificial insemination in marsupials https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7517 Sat 24 Mar 2018 08:38:30 AEDT ]]> Acrosomal integrity, viability, and DNA damage of sperm from dasyurid marsupials after freezing or freeze drying https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8081 Sat 24 Mar 2018 08:34:26 AEDT ]]> The short-term storage and cryopreservation of spermatozoa from hylid and Myobatrachid frogs https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1432 Sat 24 Mar 2018 08:28:03 AEDT ]]> Cryopreservation and storage of spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:2473 Sat 24 Mar 2018 08:27:46 AEDT ]]> Successful recovery of motility and fertility of cryopreserved cane toad (Bufo marinus) sperm https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:2805 Sat 24 Mar 2018 08:27:19 AEDT ]]> The spermatozoa of the dasyurid marsupial, Sminthopsis crassicaudata, are highly susceptible to cold shock https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11160 Sat 24 Mar 2018 08:08:32 AEDT ]]> Characterization of an L-amino acid oxidase in equine spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27633 L-tryptophan > L-tyrosine) were substrates for this enzyme, eliciting the dose- and time-dependent generation of ROS via mechanisms that were enhanced by cell death. This unexpected result was confirmed by analyses of ROS generation in subcellular sperm fractions, which again located a majority of LAAO activity to the sperm head. Equine cryopreservation medium was shown to contain sufficient quantities of aromatic amino acids to activate the LAAO system and generate ROS. The biological significance of this activity was established in an experiment in which physiological concentrations of aromatic amino acids were found to suppress sperm motility but only if dead spermatozoa were present in the same suspension. The combination of aromatic amino acids and nonviable cells was also found to enhance the levels of lipid peroxidation in live spermatozoa. These results suggest the potential significance of LAAO activity in generating the oxidative stress associated with the cryopreservation of equine spermatozoa. It is possible that inhibitors of this enzyme system may facilitate the development of modified cryostorage regimes for clinical validation in vivo.]]> Sat 24 Mar 2018 07:34:05 AEDT ]]> Emerging arguments for reproductive technologies in wildlife and their implications for assisted reproduction and conservation of threatened marsupials. https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50610 Mon 31 Jul 2023 13:05:07 AEST ]]> Integrating biobanking minimises inbreeding and produces significant cost benefits for a threatened frog captive breeding programme https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37903 Mon 21 Jun 2021 15:18:27 AEST ]]> Refrigerated storage and cryopreservation of hormonally induced sperm in the threatened frog, Litoria aurea https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54771 50%) when preserved with 15% v/v DMSO and 1% w/v sucrose; and (3) spermic urine stored at 5 ◦C retained motility for up to 14 days. Our findings demonstrate that the protocols developed in this study allowed for successful induction and recovery of high-quality spermatozoa from a threatened Australian anuran, L. aurea, providing a prime example of how ARTs can contribute to the conservation of rare and threatened species.]]> Mon 11 Mar 2024 15:07:50 AEDT ]]>